Purification of bioactive compounds from methanolic crude extracts of Zingiber Officinale and their anticancer activity against mammalian cell line
Himayoon Rashid, Umamaheswari. G and Rajakumar. R
Post graduate and Research Department of Biotechnology, Marudupandiyar College, Thanjavur-613 403, Tamil Nadu, India.
A few of the drugs are simple synthetic modifications of naturally obtained substances from medicinal plants. Ginger (Zingiber officinale), falls among the frequently used medicinal plant. The present study mainly focused on the anticancer activities of methanol fraction of Zingiber officinale. The active compounds were purified from methanolic crude extracts of Zingiber officinale (Ginger) by using TLC. Methanolic extracts showed four different bands with 0.4, 0.15, 0.43 and 0.60 retention factors respectively. In this study, seven fractions were reconstituted in methanol and subjected to column chromatography (300mm x 10 mm) on silica gel. The Gingerols was very interesting a biological activity was selected for the identification of compounds by HPLC. In LC-MS analysis, the purified column fraction in total ion chromatogram was identified. The ion mass [M+H]+2 was detected in positive mode with relative abundance. The ion 180.89 and 977.21 were determined the mass of abundant when compared with other. The cytotoxicity activity of Z. officinale methanol fraction showed against HCT-116 and DU145 cell lines. The maximum percentage (75.62 %) of inhibition was showed the methanolic fraction of Z. officinale at 320µg/ml concentration against HCT 116 cell line. . In this study revealed that the maximum (61 %) and minimum percentage (1.97 %) of inhibitions were noted in the concentrations of 320 and 5 µg/ml respectively.
